The "Efficient and Rapid Non-test Tube Plant Cloning" technique has gained international recognition, being hailed as the "Master of Green Copying." This groundbreaking technology was pioneered by Li Changkai, who has made significant contributions to plant biotechnology. Currently, he holds multiple leadership roles, including Executive Director of the China High-tech Industrialization Research Association and Chairman of Ningxia Kelvin Industrial Co. His research has led to over 30 publications, including "Plant Non-tube Efficient Rapid Propagation Technology," and he is widely regarded as a pioneer in promoting rapid propagation as a high-efficiency industry.
Developed by researcher Li Changzhen, this innovative method, known as TERNPC (Plant Non-tube Efficient Rapid Propagation Technology), represents a major advancement in both traditional seedling techniques and modern tissue culture. It has been successfully applied to nearly 700 economic plants, demonstrating its broad applicability. The technology was recognized as internationally advanced in 1995 and later valued at RMB 1.6 billion in 1999. It continues to rank among the leading global standards, with no comparable alternatives currently available.
TERNPC has received numerous awards, including the China Academy of Agricultural Sciences Technology Fair Golden Award and the National Invention Exhibition Gold Award. It has also been included in key national projects, such as the Torch Program and the State Planning Commission's high-tech industrialization initiative. Its impact on agriculture, forestry, horticulture, and medicine has been transformative, enabling efficient and large-scale propagation of valuable plants.
Despite the success of traditional methods like cuttings and grafting, which have been used for nearly 2000 years, they suffer from slow reproduction rates and limited scalability. Similarly, plant tissue culture, while effective, is costly and complex, limiting its widespread use. TERNPC addresses these challenges by using tiny explants (0.3–1.0 cm) that can be directly inoculated into simple growing media, achieving survival rates of up to 100% within days. It allows for year-round propagation, regardless of climate or location, and significantly reduces production costs.
This technology offers numerous advantages: it requires minimal investment, is easy to operate, and can be managed by trained personnel. With a propagation coefficient of 2–15 per generation, it enables the rapid multiplication of plant material. The resulting seedlings are strong, disease-free, and highly productive, making them ideal for large-scale agricultural and ecological applications.
TERNPC has revolutionized the field of vegetative propagation, offering a sustainable and economically viable solution for the cultivation of a wide range of crops. Its potential extends to the conservation of endangered species, virus-free propagation, and the development of transgenic plants. As the technology continues to evolve, it is expected to play a critical role in addressing global food security, environmental sustainability, and economic growth.
With an estimated market demand for seedlings expected to remain high, the industrialization of TERNPC presents a promising opportunity. The technology is not only cost-effective but also highly scalable, capable of generating substantial profits even at low price points. As more regions adopt this innovation, it will continue to drive progress in green industries and contribute to a more sustainable future.
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