How to use the autoclave

How to Use the Autoclave

Clicks: 5816 | Release time: 2009/10/8

^{Open the Cover} ^{. Rotate the handwheel a few turns to the left until it is turned to the top, so that the lid is fully lifted. Pull up the safety bolt on the left force column and push the beam laterally.}

² ^, ^{Â Â Â Â Â Â Â Â Â} ^{Power On} ^{. Plug in the plug and press the power switch on the control panel to the} ^ON ^{position. At this time, the undervoltage buzzer sounds.}

³ ^, ^{Â Â Â Â Â Â Â Â Â} ^{Add Water} ^. ^{Add about} ⁸ ^{liters of distilled water until the high water level (} ^HIGH ^{green light) is on.} ^. ^{If too much water is added to the inner tank, the lower valve should be opened to prevent the water from overflowing.}

⁴ ^, ^{Â Â Â Â Â Â Â Â Â} ^Stacking ^. ^{There should be a certain gap between the packages. Do not block the safety valve with the sterilization bag.}

⁵ ^, ^{Â Â Â Â Â Â Â Â Â} ^Seal ^. ^{Make sure the lid and the pot are fully closed. When the interlocking light (green light) is on, it indicates that the container is properly sealed.}

⁶ ^, ^{Â Â Â Â Â Â Â Â Â} ^{Set Temperature and Time} ^{. Press the ENTER button once to set the temperature. After setting, press the ENTER button twice to confirm the temperature. Then press again to start setting the time. The first two digits represent the hour, and the last two digits represent the minute. After the settings are complete, press the confirmation button twice to finish the process.}

⁷ ^, ^{Â Â Â Â Â Â Â Â Â} ^Sterilize ^{. After the temperature and time have been set, the heat lamp (green light) on the control panel will turn on. During sterilization, you can slightly rotate the drain valve to the left (to the right for drainage) to release a small amount of steam, which helps equalize the temperature inside the chamber.}

⁸ ^, ^{Â Â Â Â Â Â Â Â Â} ^Exhaust ^{. After the sterilization cycle is complete, turn off the power switch, unplug the device, and wait until the pressure gauge reads zero. Then open the safety valve or the drain and exhaust valve to release the remaining pressure in the chamber.}

⁹ ^, ^{Â Â Â Â Â Â Â Â Â} ^{Open the Cover}

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Lactobacillus Johnsonii

Lactobacillus johnsonii, a gram-positive bacterium, belongs to phylum Firmicutes, class Bacilli, order Lactobacillales, family Lactobacillaceae, and genus Lactobacillus, which is facultatively anaerobic and sometimes microaerophilic, exhibiting poor growth under aerobic conditions but better growth at reduced oxygen pressure. L. johnsonii was first isolated from human blood, with other primary sources including humans, mice, and pigs (Pridmore et al., 2004; Vazquez-Munoz et al., 2023; Zhang et al., 2019). The health-associated benefits of L. johnsonii are known for its antibacterial properties, inhibiting pathogenic bacteria such as Helicobacter pylori and Staphylococcus aureus (Aiba et al., 2019; Rosignoli et al., 2018). It is also viewed as a substitute for antibiotics in poultry feed due to its ability to suppress Clostridium perfringens (Gervasi et al., 2014). Multiple strains of L. johnsonii contain bile salt hydrolase (BSH) (Boucard et al., 2022), which has been reported to have anti-Giardia lamblia activity and reduce cholesterol (Allain et al., 2018; Zhu et al., 2022). Additionally, L. johnsonii FI9785 can produce extracellular polysaccharides (EPS), which enhances its colonization ability (Mayer et al., 2020). In clinical research, L. johnsonii can serve as an adjuvant to help alleviate type 1 diabetes and allergic rhinitis (Lue et al., 2012; Wang et al., 2022). L. johnsonii exhibits significant antimicrobial potential and has been proven to be effective in livestock farming as a substitute for antibiotics.

Probiotics, Johnsonii, extracellular polysaccharides

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How to use the autoclave

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