The growth and development of earthworm can be divided into two stages, the mycelium growth stage and the sclerotium formation stage. The mycelial growth stage is mainly the hyphae absorbing moisture and nutrients from the pine wood, and multiplying the vegetative mycelium. When the mycelium grows more and more, it becomes a sclerotia, and when the sclerotia stage is formed, the cultivation is called the scab phase. Artificially cultivated alfalfa is currently commonly used as a cultivation method for the cellar cultivation method. First, the pure alfalfa strain should be cultivated, and then the digging can be performed again.
Cultivation of pure bacilli
(1) Preparation of a culture medium for quail species:
Potato-agar medium is usually used. The formula is 250 g potato, 50 g sucrose, 20 g Qiong 8 fat, 3 g urea, and 1000 ml of water. Prepare according to conventional methods and adjust the pH to 6-7. , Dressing, sterilizing, making it a slant medium. Separation and Inoculation of Pure Bacteria: Select fresh, thin skin, white flesh, tight, fragrant mature sclerotium nucleus as a strain, wash it first with water, and then move it into the inoculation box; Then, rinse with distilled water or cold water several times, dry with filter paper, cut with a knife, pick up a small piece of central white meat, place it on the slope of the culture medium, and then put it into a 25-30 degree incubator or culture. The box is cultured for 5-7 days. When the white mycelium grows on a slant, it is a pure breed.
(2) After the successful cultivation of the purebred mother of the original species can not be directly used for production, but also to expand the breeding and breeding of the original species. The original culture medium formula: 55% of pine wood blocks, 20% of pine wood chips, 20% of rice bran or wheat bran, 4% of sucrose, 1% of gypsum powder; add appropriate amount of water, and adjust PH value to 5-6. Preparation method: first wood chips, rice bran, gypsum powder and mix; add the water to dissolve the sugar, put the pine wood block boil for 30 minutes, until the pine wood block fully absorb the sugar liquid, remove; then the crumbs, rice sugar; gypsum powder Pour into the liquid, mix well; then add the wood, mix well, and use the fingers to squeeze the water for the degree. Then it is packed in a 500-ml wide-mouth bottle, sterilized, and can be seeded after cooling. Inoculation method: Under aseptic conditions, the mother strains of soybean size were selected from the parent species and placed in the center of the original species of the wide-mouth bottle, which was the original species.
(3) Cultivation of cultivated species Culturing culture medium consists of wood 66%, pine wood chips 10%, wheat bran or fine mash 21%, glucose 2%, gypsum powder 1%, urea 0.4%, calcium superphosphate 1% , add water, adjust the PH value of 5-6. The preparation method is the same as above. The mycelium in the original species was received in a wide-mouth bottle with culture material, and the mycelium covered the whole bottle in about 30 days. One bottle of pure seed can be sorted by 5-8 bottles, one bottle of original can be bred by 60 bottles, and one bottle of cultivar can be connected by 2-3 cellar.
Selection of cellar and pine wood selection of deep and loose soil, well-drained sandy loam, sunny slopes and uncultivated raw land, woodland, winter will cellar dug good, cell length 80 cm, width 45 cm, depth 30 In centimeters, the distance between the pit and the pit is 30 cm, and a drainage ditch is opened.
Using pine wood as a cultivation material, in the lunar calendar in December, the pines were cut down, the tree shrews were cut, the layered skins were cut off, the xylem was exposed, and the ribs were peeled and sawn into a 60-80 cm long section of wood. After stacking and drying, they were dried. After 40 days, there is no turpentine secretion, and the wood heart can be inoculated after drying.
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