Separation principle of ultracentrifuge

The separation principle of the ultracentrifuge is that when a solution containing a polymer or a macromolecule is separated from the gravity by 250,000 times, the molecules are separated from each other, and the pure solvent remains above the interface. This interface moves to the lower part of the container at a certain speed. . If the molecular weight of the solute is not uniform and the concentration gradient at this interface is not uniform, those with a low molecular weight will fall behind the macromolecule. This interface can be observed with an optical instrument to accurately determine the sedimentation rate, and the sedimentation rate of each component is related to its molecular weight, so that the molecular weight of each component can be calculated. The ultracentrifuge not only separates the colloidal particles, but more importantly it measures the sedimentation rate, average molecular weight and weight distribution of the colloidal particles, thus playing a major role in colloidal chemistry studies (especially lyophilic colloids).

Environmental protection Centrifugal separation is commonly used in: 1 centrifugal filtration, the liquid is removed from the slurry by means of centrifugation, the slurry is introduced into a fast-rotating basket, the solid remains on the porous network, and the liquid is centrifuged from the filter cake. Extrusion: Or use the centrifugal force in the rotator to separate the light and heavy materials, and the heavy material flows away through the nozzle in the form of a thick slurry. Commonly used equipment is a centrifugal filter. 2 Centrifugal sedimentation, the suspended solids move to or away from the center of rotation under centrifugal force, so that they can be collected in one area and removed, which can reduce the precipitation time of the particles from several hours to several minutes. The commonly used equipment is a centrifugal settler. 3 Centrifugal trapping, used to separate 0. 1 } 1000 micron small particles from soot and air flow, is one of the effective means to control air pollution. Commonly used equipment is a centrifugal trap, also known as a particulate collector, a cyclone.

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