PacBio RS II Sequencer revolutionizes HLA detection typing technology [innovative skills]

Once the PacBio RS II sequencing system is introduced, its long read length, single-molecule base resolution and GC-free preference are highly regarded by researchers, especially in the field of HLA detection and classification, and the internationally authoritative HLA detection classification. The agency has introduced PacBio RS II sequencing technology to develop related services or kits, which has spurred PacBio's climax in HLA research applications.

HistoGenetics Introduces PacBio RS II Sequencing System

HistoGenetics and Pacific Bioscience announced on April 29, 2014 that, in view of the PacBio sequencing platform capable of measuring the full length of the HLA gene, thereby providing a more accurate advantage of HLA typing, the purchase of two PacBio RS II systems replaced the previous Sanger One generation of sequencing was used for HLA typing. HLA is a highly polymorphic allogeneic antigen containing tens of thousands of alleles to encode antigenic proteins to recognize foreign antigens. Accurate HLA typing is very important for organ transplantation, autoimmune disease related research, drug hypersensitivity studies, and the like. Previous Sanger and NGS short-sequence sequencing methods were not accurate enough for HLA typing. In order to obtain accurate typing results, additional costs were required. The emergence of PacBio long sequence sequencer provided a better solution for HLA accurate typing.

Dr. Nezih Cereb, CEO and founder of HistoGenetics, said in an interview: "As a leader in the HLA typing market, we are always looking for analytical techniques that can accurately target complex regions of the genome. The PacBio technology platform, by means of The industry's leadership in terms of read length, accuracy and fast sequencing time is exactly what this market needs, and we are eager to use the PacBio RS II sequencer to discover more information in the HLA region."

Anthony Nolan Research Center introduces PacBio RS II sequencing system

The Anthony Nolan Research Center and PacBio announced on May 13 that the Anthony Nolan Research Center continues its revolutionary record of technological innovation by acquiring two sets of PacBio RSII systems to achieve full HLA geneization. Long single-molecule, real-time sequencing.

According to the Anthony Nolan Research Center, the introduction of the PacBio system is based on the longest read length and highest consistency of the PacBio RSII system. With the PacBio RSII system, the Anthony Nolan Research Center will provide unparalleled detail and precision in organizing your parting services.

Professor Steven Marsh, Head of Bioinformatics, Anthony Nolan Research Center: “From our institutional perspective, the Anthony Nolan Research Center has always been a technologically advanced institution. The introduction of PacBio single-molecule real-time sequencing technology will help us achieve the allelic level. The classification of the highest resolution information in the classification, we can carry out accurate HLA typing in tissue transplantation and other applications, achieving a higher success rate in bone marrow and blood tumor cell transplantation."

GenDx and Pacific Bioscience collaborate to develop HLA full-length gene sequencing related products

GenDx and Pacific Bioscience announced on June 19, 2014 that GenDx, a pioneer based on second-generation sequencing HLA typing, will collaborate with Pacific Bioscience to develop and provide full-length HLA gene sequencing related products.

High-resolution HLA typing and HLA full-length gene sequencing enable accurate discrimination between HLA alleles, even small differences. Specific HLA typing is critical for studies of immune-related diseases, drug hypersensitivity studies, and other applications. Therefore, more and more people are interested in clarifying the characteristics of the entire HLA gene. GenDx currently offers HLA class I full-length amplification reagents and will offer Barcode primers based on the PacBio RS II DNA sequencing system in its NGSgo product line. These kits will help researchers complete a mixed sample of full-length HLA I genes from different sources in the same SMRT sequencing well.

Dr. Wietse Mulder, General Manager of GenDx, commented: "The full-length HLA gene sequencing method was originally developed at GenDx based on the Sanger sequencing method. This method has also been sequenced and proven HLA in tens of thousands of samples. Both alleles can be amplified. We are very excited that PacBio's SMRT sequencing technology can now fully perform genotyping and obtain very accurate typing results, and we believe this innovative technology will be available in the HLA field. Many organizational parting programs."

Dr. Mats Bengtsson, Director of the Transplantation Immunology Laboratory, commented: "We believe that PcBio's long length combined with its rapid sequencing time will cause waves in HLA typing applications. And this full-length based, accurate score Type results and the ability to identify new alleles are unprecedented."

HistoGenetics is a world leader in HLA genotyping. As a pioneer in this field, HistoGenetics has provided a number of services for donor registration agencies, donor centers, cord blood banks, transplantation centers, HLA typing laboratories, and pharmacogenomics applications.

The Anthony Nolan Research Center is a UK charity dedicated to the research of patients with leukemia and hematologic malignancies. Founded in 1974, the agency is dedicated to serving patients who require bone marrow and stem cell transplantation. For a successful transplant experiment, the donor and patient receptor HLA genotypes need to be perfectly matched. HLA genotype identification first requires PCR amplification of the region of interest, and a large number of copies are cloned. Then HLA gene scores can be performed by SSOP (Sequence specific oligonucleotides), SSP (Sequence specific primers), and SBT (Sequence based typing). type.

GenDx is a Dutch molecular diagnostics company that develops and markets in vitro diagnostic tests and services, analytical software sales and technical training, and is a pioneer in the field of sequencing-based organ transplantation. GenDx provides worldwide Sanger sequencing supporting analysis software and reagents for transplant laboratories, and later added various software and reagent accessory products based on the second-generation sequencing platform to sequence the entire sequencing process.

The Tianjin Biochip uses the PacBio platform to sequence the genomes for bacteria up to 70% of the GC content of the soil. Only two cells were sequenced, and more than 900 Mb of sequencing data was obtained to achieve 100× of the genome of the bacteria. Sequencing coverage. The genomic map of the bacterium was obtained by PacBio data De novo splicing; we then performed site correction using the Illumina platform to obtain a highly accurate genomic completion map.